cox 2  (Boster Bio)

Journal: BMC pulmonary medicine

Article Title: Ciprofol prevents ferroptosis in LPS induced acute lung injury by activating the Nrf2 signaling pathway.

doi: 10.1186/s12890-024-03415-w

Figure Lengend Snippet: Fig. 2 ciprofol reduced LPS induced ferroptosis and increased antioxidant ability in mice. A-C Activities of malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) in the murine lung tissues (n = 6 per group). D-G GPX4, 4-HNE and PTGS2 expressions in lung tissues detected by western blot (n = 3 per group). H Expression level of Fe2+ in murine lung tissues (n = 6 per group). I 4-HNE expressions in lung tissues detected by im mumohistochemical staining. J Transmission electron microscopy images of representative mitochondrial structures (scale bar, 1.0 μm). K quantitative analysis of 4-HNE-positive area (n = 6 per group). Data are presented as Mean ± SD.Statistical analysis was performed using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Protein samples in identical amounts from each group were subjected to 10% SDS-PAGE and then placed onto a PVDF membrane.Furthermore, the membranes were blocked with 5% skim milk in TBST at 37 °C for 1 h and incubated overnight with primary antibodies against Nrf2 (1:5000, Proteintech, 16396-1-AP), GPX4 (1:10000, Abcam, ab125066), 4-HNE (1:500, BIOSS, bs-6313R), PTGS2(1:800, BOSTER, BM4419), SLC7A11 (1:500, Abcam, AB307601), and β-actin (1:2000, ZSGBBIO, TA-10) at 4 °C.

Techniques: Western Blot, Expressing, Staining, Transmission Assay, Electron Microscopy

Journal: BMC pulmonary medicine

Article Title: Ciprofol prevents ferroptosis in LPS induced acute lung injury by activating the Nrf2 signaling pathway.

doi: 10.1186/s12890-024-03415-w

Figure Lengend Snippet: Fig. 4 ciprofol reduced LPS induced ferroptosis and increased antioxidant ability in MLE12 cells.A ROS production detected by immunofluorescence and its quantification analysis (n = 3 for each group). B-D Activities of malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) in MLE12 cell s (n = 6 for each group). E,K Representative immunofluorescence images of 4-HNE, GPX4 and the quantification analysis in MLE12 cells (n = 3 for each group). F-I GPX4, 4-HNE and PTGS2 expressions in MLE12 cells detected by western blot (n = 3 for each group). J Expression level of Fe2 + in MLE12 cells (n = 6 for each group). Data are presented as Mean ± SD. Statistical analysis was performed using one-way ANOVA.*p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Protein samples in identical amounts from each group were subjected to 10% SDS-PAGE and then placed onto a PVDF membrane.Furthermore, the membranes were blocked with 5% skim milk in TBST at 37 °C for 1 h and incubated overnight with primary antibodies against Nrf2 (1:5000, Proteintech, 16396-1-AP), GPX4 (1:10000, Abcam, ab125066), 4-HNE (1:500, BIOSS, bs-6313R), PTGS2(1:800, BOSTER, BM4419), SLC7A11 (1:500, Abcam, AB307601), and β-actin (1:2000, ZSGBBIO, TA-10) at 4 °C.

Techniques: Immunofluorescence, Western Blot, Expressing

Journal: BMC pulmonary medicine

Article Title: Ciprofol prevents ferroptosis in LPS induced acute lung injury by activating the Nrf2 signaling pathway.

doi: 10.1186/s12890-024-03415-w

Figure Lengend Snippet: Fig. 5 ciprofol reduced LPS induced ferroptosis through Nrf2-dependent pathways in MLE12 cells. A Cell apoptosis detected by flow cytometry. B Cell viability determined by CCK8 assays (n = 6 for each group). *P < 0.05 vs.LPS group; #P < 0.05 vs.LPS + Cip group. C Expression level of Fe2+ in MLE12 cells. D-I GPX4, 4-HNE, PTGS2, Nrf2 and SL7A11 expressions in MLE12 cells detected by western blot (n = 3 for each group). J Representative immunofluorescence images of Nrf2 and its quantification analysis in MLE12 cells (n = 3 for each group). K Apoptotic rate measured by Annexin V-FITC/PI flow cytometry (n = 3 for each group). Data are presented as Mean ± SD. Statistical analysis was performed using one-way ANOVA.*p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Protein samples in identical amounts from each group were subjected to 10% SDS-PAGE and then placed onto a PVDF membrane.Furthermore, the membranes were blocked with 5% skim milk in TBST at 37 °C for 1 h and incubated overnight with primary antibodies against Nrf2 (1:5000, Proteintech, 16396-1-AP), GPX4 (1:10000, Abcam, ab125066), 4-HNE (1:500, BIOSS, bs-6313R), PTGS2(1:800, BOSTER, BM4419), SLC7A11 (1:500, Abcam, AB307601), and β-actin (1:2000, ZSGBBIO, TA-10) at 4 °C.

Techniques: Flow Cytometry, Expressing, Western Blot, Immunofluorescence

Journal: BMC pulmonary medicine

Article Title: Ciprofol prevents ferroptosis in LPS induced acute lung injury by activating the Nrf2 signaling pathway.

doi: 10.1186/s12890-024-03415-w

Figure Lengend Snippet: Fig. 6 The inhibition of ciprofol on ferroptosis was abolished in Nrf2 KO mice. A Representative HE staining images and lung injury score of the lung tissues (n = 6 for each group). B Proinflammatory cytokine IL-1β, IL-6, and TNF-α levels in lung tissues (n = 6 for each group). C Activities of MDA, GSH, SOD and Fe2 + content in lung tissues (n = 6 for each group). D GPX4, 4-HNE expressions detected by immumohistochemical staining and the quantification analysis in lung tissues (n = 3 for each group).E Nrf2 expressions detected by immumohistochemical staining and the quantification analysis in lung tis sues (n = 3 for each group). F GPX4, 4-HNE, PTGS2, Nrf2 and SL7A11 expressions detected by western blot in lung tissues (n = 3 for each group). Data are presented as Mean ± SD. Statistical analysis was performed using t-test. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Protein samples in identical amounts from each group were subjected to 10% SDS-PAGE and then placed onto a PVDF membrane.Furthermore, the membranes were blocked with 5% skim milk in TBST at 37 °C for 1 h and incubated overnight with primary antibodies against Nrf2 (1:5000, Proteintech, 16396-1-AP), GPX4 (1:10000, Abcam, ab125066), 4-HNE (1:500, BIOSS, bs-6313R), PTGS2(1:800, BOSTER, BM4419), SLC7A11 (1:500, Abcam, AB307601), and β-actin (1:2000, ZSGBBIO, TA-10) at 4 °C.

Techniques: Inhibition, Staining, Western Blot